In Vitro Fertilization (IVF)

Founders of IVFThis procedure was first successfully used in humans in 1977 in England by Drs. Steptoe and Edwards. The scientific importance of this discovery was recently recognized with the awarding of the Nobel Prize in Medicine to Dr. Edwards. To date, millions of babies have been delivered worldwide as a result of this treatment. The procedures to achieve IVF pregnancy have become increasingly simpler, safer, and markedly more successful.

To achieve pregnancy as a result of IVF, several steps are necessary and each one of them will be described in detail to help you better understand the treatment as it will occur.

  1. Hormonal suppression or ‘down-regulation’ of your menstrual cycle with birth control pills or other drugs.

  2. This process allows us to take charge of your ovaries so that there is no follicular growth occurring at all, and helps to synchronize your follicles to allow multiple eggs to develop at once.  Depending on the medications used, there is a chance you will temporarily undergo some of the symptoms of menopause, but these symptoms will go away during the stimulation phase.  This will then make sure that during the next stage of the treatment we have full control of the stimulation.

     

  3. Stimulation of the ovaries to produce several eggs.

  4. During this phase of your treatment cycle you need to continue the drugs that ‘switch off’ your bodies own control of your ovaries and also start taking medication that will stimulate them.  The reason for continuing the suppression medications is to stop the chance of you ovulating before we get to the egg retrieval, so it is very important that you keep taking those drugs.  The stimulation is carefully controlled to allow for the most optimal follicle recruitment possible – the dosage is decided based on all of the workup that you did before starting the cycle.

    An ultrasound image of stimulated ovary with multiple follicles visible (dark spots)

    You will be monitored during this phase of your treatment to make sure that you are responding appropriately by doing blood draws to check your estradiol levels and by vaginal ultrasound scans to measure the follicle sizes.  There are times when we either increase or decrease the drug dosage depending on what we see, and you will be informed accordingly.

    It is very important that you continue to follow your calendar very closely during this period and to follow any and all changes that we may make depending on your response.  You will need to make sure that you are flexible to allow for appointments at short notice and that you are available via phone each day.

     

  5. Retrieval of the eggs from the ovaries

  6. Once it has been determined that your follicular growth is optimal then we will schedule your egg retrieval.  This is achieved by the administration of a carefully timed ‘trigger shot’ that allows for the final maturation of the follicles and readies the eggs for release.  The exact nature of the shot depends on your response to stimulation and you will be given full instructions at the time.  You will be given a precise time for the trigger and it is very important that you stick with this time.

    You will be under deep sedation anesthesia for the egg retrieval procedure, under the care of a MD anesthesiologist. The anesthesiologist will keep you asleep for the procedure. You will be given instructions as to when we need you to arrive at the clinic and how to prepare for the case.

    The retrieval itself utilizes the same vaginal probe ultrasound that was used during the monitoring phase of treatment, but with a guide attached to it.  That guide allows a needle to be passed through the back of the vagina and into the ovary under direct visualization.  The needle itself allows both the contents of the follicle to be aspirated, and then media to be pushed back into the follicle to ‘rinse it out’.  Then the fluid is passed to the embryologist in order to look for the egg.  They are very visible within the fluid from the follicle, and they can be separated and placed into culture media.

    A diagram depicting the the egg retrieval process during IVF

    This step also utilizes the IVF Witness RFID tagging system which allows us to allocate your name to the tag that has been placed on the dish for chain of custody monitoring of the identity of all samples. This ensures that the right eggs, sperm, and embryos are used for every procedure we do.

     

  7. Fertilization of the eggs and cultivation of the embryos in the laboratory

  8. Once we get the eggs back into the laboratory they are placed into new culture media and are placed into the incubator.  We also need to process the sperm sample that we are going to be using for the insemination later that day.  In most cases we will use an ejaculated sample that has been produced at some point during the morning.  The sample is processed to remove all the seminal plasma and to concentrate the motile sperm.  On occasion we can use a frozen sample; the process is very similar in that the sample is washed, this time to remove the cryoprotectants as well as the seminal plasma.

    The insemination procedure is carried out during the afternoon – timed to take place 6-8 hours after the scheduled retrieval start time.  There are two different methods that can be used to inseminate the eggs, and the decision of which to use is dependent on the quality of the semen sample and also the couple’s history.  For the most part we will have made the decision prior to the cycle as to which method we will be using, either conventional IVF when the sperm is simply added to the dish with the eggs, or Intra Cytoplasmic Sperm Injection (ICSI) where individual sperm are injected into each egg.

    The morning after the egg retrieval (Day 1) is when we check the eggs to see which ones have fertilized, and you will be given a call that morning to update you about the status.  After this point the embryos are left in the incubator, evaluated at specific times and moved into fresh culture media as their developmental requirements change.

    Fert check IVF embryo

    A fertilized embryo

    All this leads up to the point at which they are going to be transferred into the uterus, on Day 2, 3, 5 or in some cases 6.  This decision is guided by the number and quality of the available embryos, the age and reproductive history of the patient, and also the current guidelines set down by the American Society for Reproductive Medicine (ASRM).  All of this information will be discussed with you before making any decisions about your embryos.  It is important to be flexible during this period as your scheduling may change depending on the speed of development, quality, and number of your embryos.

     

  9. Placement of the embryos into the uterus

  10. The embryo transfer procedure is the same regardless of which day of culture it happens.  Once we have all decided on the number of embryos that are going to be replaced then we will make sure that we have selected the most appropriate ones to transfer based on their development and growth over the culture period.  If it is required the embryos will be hatched prior to the transfer (using a targeted laser to weaken the zona pellucida – the outer covering of the embryo) and then they will be placed into a culture dish ready for the transfer itself.

    The first part of the transfer is where the physician finds the best route into the uterus through the cervical canal, and places the outer sheath of the catheter into your cervix under abdominal ultrasound guidance.  While this is happening the embryologist will be waiting in the laboratory and will bring the embryos through into the procedure room only when he or she knows that the doctor is ready.  The embryos are then quickly loaded into the catheter, that is threaded through the outer sheath that is already in place and the embryos are expelled into the uterus.  After they have been transferred we will carefully check the catheter to make sure that they have not been left behind before the procedure is complete.

    After the transfer you will be asked to remain lying down for about 15-20 minutes and then you are free to go about the rest of your day.  You will be given full follow up instructions regarding what activities should be avoided and what new medications you will take.

     

  11. Cryopreservation of any remaining high quality embryos

  12. After your transfer it is possible to cryopreserve (freeze) any good quality embryos for future use.  We generally will only freeze embryos at the blastocyst stage, but they can be frozen at other stages if it is deemed appropriate.  The reason that only good quality embryos are frozen is because the freezing and thawing procedure can place some stress on the embryos and poorer quality ones do not do as well.

    All of the procedures that are carried out in the laboratory here at Overlake Reproductive Health utilize the IVF Witness system (see relevant webpage).  This allows us to use Radio Frequency Identification tags (RFID) on every single culture dish or test tube to safely identify the contents – eggs, sperm, or embryos – as the ones that belong to you.

    Watch the video to learn more about IVF at ORH

    IVF offers hope for couples who might not be able to conceive through natural means alone. Technological advancements and constantly improving techniques continue to make IVF a safer, simpler, and more successful procedure. You can read more about our world class IVF success rates. If you would like to learn more about IVF or want to discuss your options with one of our highly trained fertility specialists please contact us.