The second morning urine is the best time to test – roughly between 10 am and noon. The ovulation predictor test looks for a hormone called LH or luteinizing hormone in your urine. LH hormone surges 24 to 36 hours before you ovulate, but the surge may occur first thing in the morning and it can take 4 hours for the hormone to come out in your urine, so first morning urine is not the best time to test.
If you have 28 day cycles, test between days 10 to 18. If your cycles are longer but regular, like 32 days, start 32 minus 28 or 4 days later – days 14 to 22. Day 1 is the first day of your period. See a Fertility specialist (Reproductive Endocrinology and Infertility or REI MD) if you’re not pregnant after up to a year of trying and you’re 34 years old or under, or not pregnant after six months of trying if you’re 35-39. See a REI soon if you have irregular cycles, or you’re 40 or older.